Localization of Multiple Hydrogels with Multicube Platform Spatially Guides 3d Tissue Morphogenesis In Vitro

Adv. Mater. Technol. 2023, 8, 2201660 https://doi.org/10.1002/admt.202201660 In the original version of this article, there were errors in concentration units within article and figure captions. These made by editorial team during production process. On other hand, supplementing Matrigel with 2.5 × 10−9 M heparin-binding EGF-like growth factor (HBEGF), an EGFR-binding ligand a higher affinity to Matrigel,[35,36] successfully generated NHBE branching under EGF(–) medium over 3 days (Figure 4A, 4th column) To compare retention factors Matrigel, clusters also cultured (i) normal GFR-Matrigel, i.e., or control Matrigel; (ii) GFR-Matrigel premixed EGF at final concentration, EGF(+) (iii) human recombinant (HBEGF; E4643, Sigma) HBEGF(+) Matrigel. three conditions submersion. For HBEGF localization, was ice bath. Then, it washed once IF solution stained 600 DAPI DPBS for 1 h. After blocking, samples from tissue branching-pattern model 50 10−6 phalloidin (PhenoVue Fluor 488; CP24881, PerkinElmer) together The correct captions Figure 2 4 are as follows: 2: unit design MultiCUBE effect physical parameters on solution-trapping ability. A) A cubic straight frames failed trap hydrogel inside whereas L-shaped without dripping shifting. B) frame lacked sufficient barriers counter against weight (F↓) countered providing surface extension along dashed outline adhesion (F↑). Different conformations trapped solutions indicated different C) no hanging base full trapping. D) stable partial trapping; E) F) spreading trappings. G–I) varied across inner-cube space (S) window-frame size (W), well wettability. symbol each (S,W) grid represented occurrence conformation: X (dripping), (spreading), O (hanging base), (no base). If unrepeatable, example, particular one dripping, two hanging-base four would be symbolized X. number repeats is n = 6, 7 5 untreated, hydrophilic, hydrophobic frames, respectively S1). 4. Localization hydrogels resulting morphogenesis. Epidermal (EGF) presents standard drove tissues, removing resulted nonbranching tissues. Although generate branches, (HBEGF) extensive spheroids. Scale bar: 500 µm. B,C) only 3rd guided development tip part tissues culture. D,E) apparent branch density total length all significantly than body part. nonlocalizing culture where exposed Matrigels medium, G,H) significant difference found between parts Data presented scattered boxplots (x: outlier, solid line: median, dash mean). Statistical significances evaluated one-way ANOVA Tukey–Framer post-hoc test (n.s.: difference, *p < 0.05, **p 0.01, ***p 0.001). corrections do not affect results conclusions work. office apologizes inconvenience caused.